Background: Identification and treatment of children with latent tuberculosis infection (LTBI) is essential in tuberculosis (TB) control strategy. TB screening is only provided for children with TB contacts or for any United States (US) bound immigrants. The aim of this study was to determine the prevalence of LTBI and its associated risk factors among children with US visa applications in Korea. Methods: The medical history, vaccination history and results of TB screening of US visa applicant children who visited Yeouido St. Mary’s Hospital, The Catholic University of Korea were collected. TB screening included chest radiograph and tuberculin skin test. Results: Of 1664 study subjects, 91 (5.5%) had LTBI. The proportions of children with LTBI significantly increased with age. The proportions of children with LTBI were significantly higher among those born in high TB burden foreign countries than among those born in Korea and in low TB burden foreign countries. The individuals who were not immunized with BCG were more frequently affected by LTBI than those immunized with BCG. There were higher proportions of children with LTBI who were diagnosed with asthma in comparison to those without asthma. On binary logistic regression analysis, born in high TB burden foreign country presented highest adjusted odds ratio, was most associated significant risk factors of LTBI. The increase of age, no BCG vaccination and previous diagnosis of asthma were also significant risk factors of LTBI. Conclusions: Birth in a high TB burden foreign country was the strongest risk factor of LTBI among US visa applicant children in Korea, the attention is needed to these children.